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Article Title: Toxicity Effects of Diesel on Plankton Cell Population and Diversity Using Algal Bloom Test at Different Time Intervals.


The effects of toxicant on plankton cell of cultured algal bloom in a bioassay test were investigated. The medium used for the cultivation of the Algal bloom was formulated using the following in g/l; KNO3 0.132, silicate 0.066, NaHPO4 0.066, EDTA 0.066. The medium was sterilized by autoclaving at 121°C for fifteen minutes and allowed to cool. 1ml of sea water was inoculated into the sterile medium. The conical flask was kept at room temperature, but exposed to sunlight. This was monitored until blooming occurred. Various concentrations of the diesel in mg/l (0, 0.01, 0.1, 1.0, 10, 100 and 1000) were introduced into different bloom vials where plankton cells were enumerated using centrifuge plankton counting chambers and identified using standard keys for total count after exposure for different time duration. The result indicates that diesel had significant impact on cell count (F6, 35 = 152.7, P < 0.001). Zero concentration had limited effect on cell count (257 ± 5.0) whereas concentration > 0.1 mg/l had adverse effect on cell count (65 ± 2.0). Time of treatment had no effect on cells count (F5, 35 = 0.086, P = 1.0). However, Tukey HSD test indicates that 48, 72 and 96 hours treatments had the most impact on cell count. This result implies that the interaction of concentration and time has a devastating effect on phytoplankton cells. This study thus shows the effect of petroleum products on our water ways due to oil exploration and exploitation activities in the Niger Delta, Nigeria.